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    • Insights into the role of three Endonuclease III enzymes for oxidative stress resistance in the extremely radiation resistant bacterium Deinococcus radiodurans 

      Rollo, Filipe; Martins, Guilherme D.; Gouveia, André G.; Ithurbide, Solenne; Servant, Pascale; Romão, Célia V.; Moe, Elin (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-09-12)
      The extremely radiation and desiccation resistant bacterium Deinococcus radiodurans possesses three genes encoding Endonuclease III-like enzymes (DrEndoIII1, DrEndoIII2, DrEndoIII3). In vitro enzymatic activity measurements revealed that DrEndoIII2 is the main Endonuclease III in this organism, while DrEndoIII1 and 3 possess unusual and, so far, no detectable EndoIII activity, respectively. In ...

    • The three Endonuclease III variants of Deinococcus radiodurans possess distinct and complementary DNA repair activities 

      Sarre, Aili; Stelter, Meike; Rollo, Filipe; De Bonis, Salvatore; Seck, Anna; Hognon, Cecilia; Ravanat, Jean-Luc; Monari, Antonio; Dehez, Francois; Moe, Elin; Timmins, Joanna (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-28)
      Endonuclease III (EndoIII) is a bifunctional DNA glycosylase that removes oxidized pyrimidines from DNA. The genome of Deinococcus radiodurans encodes for an unusually high number of DNA glycosylases, including three EndoIII enzymes (drEndoIII1-3). Here, we compare the properties of these enzymes to those of their well-studied homologues from E. coli and human. Our biochemical and mutational data, ...